Fig. 1: Lentiviral delivery of the CRISPR knockout library identified genes that markedly accelerate ICC formation in SPC mice. | Cell Death & Disease

Fig. 1: Lentiviral delivery of the CRISPR knockout library identified genes that markedly accelerate ICC formation in SPC mice.

From: The heterogeneity of signaling pathways and drug responses in intrahepatic cholangiocarcinoma with distinct genetic mutations

Fig. 1: Lentiviral delivery of the CRISPR knockout library identified genes that markedly accelerate ICC formation in SPC mice.The alternative text for this image may have been generated using AI.

A Summary of tumor number collected from each mouse. Livers developed tumors at 3–4 months after injection with pX330 containing the corresponding sgRNA. sgSmad4 was included as a negative control. B, C Kaplan‒Meier survival plots show survival probability with different gene expression. D Mutation rates of the TP53, FBXW7, INPPL1, TGFBR2, and CUL3 genes among CC patients based on the COSMIC database. E Livers injected with pX330 containing sgInppl1, sgTgfbr2 or sgFbxw7 developed ICC tumors. H&E staining and immunohistochemistry (IHC) staining for AE1 and Hep par1. Scale bar = 20 μm.

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