Fig. 2: The identified gene mutation promotes cancer progression in vitro and in vivo. | Cell Death & Disease

Fig. 2: The identified gene mutation promotes cancer progression in vitro and in vivo.

From: The heterogeneity of signaling pathways and drug responses in intrahepatic cholangiocarcinoma with distinct genetic mutations

Fig. 2: The identified gene mutation promotes cancer progression in vitro and in vivo.

A Cell proliferation assay in transfected cells. Cell viability was examined with Alamar Blue; the fluorescence intensity was normalized to the values on Day 0. B Tumor volume measured at different time points in nude mice bearing 273cc cells transplanted with different sgRNAs. (n = 6–9 in each group). C, D H&E staining and IHC staining for AE1, Hep par1 and PCNA in 273cc cell transplanted tumors with different mutations. Scale bar = 20 μm, inset scale bar = 10 μm.

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