Fig. 5: Different pathways regulated by CRISPR/Cas9-induced gene mutation in ICC cells.

RNA-seq analysis revealed that sgCul3 (A, B), sgFbxw7 (C, D), sgInpppl1 (E, F), sgTgfbr2 (G, H), and sgTrp53 (I, J) induce different pathway changes in CCA cells. ***p < 0.001. The expression levels were normalized to control group. (n = 3 for each group).