Fig. 2: CMI initiated microglial trained immunity in the contralateral cortex.
From: Cortical microinfarcts potentiate recurrent ischemic injury through NLRP3-dependent trained immunity
Representative GFAP and Iba1 IHC staining (A) and quantitative analysis for Iba-1 (B–D) and GFAP- (E) positive astrocytes around the contralateral cortex following CMI, n = 12 mice per group. One-way ANOVA F4, 55 = 2.808, p = 0.0342. Sham vs. 1w: p = 0.2748; Sham vs. 2w: p = 0.8075; Sham vs. 3w: p = 0.9621; Sham vs. 4w: p = 0.9515 (B); F4, 55 = 34.01, p < 0.0001; Sham vs. 1w: p < 0.001; Sham vs. 2w: p < 0.001; Sham vs. 3w: p = 0.0142; Sham vs. 4w: p = 0.9215 (C); and F4, 55 = 9.049, p < 0.0001; Sham vs. 1w: p = 0.0008; Sham vs. 2w: p = 0.0017; Sham vs. 3w: p = 0.0540; Sham vs 4w: p = 0.9999 (E) with Tukey’s correction. Scale bars, 25 μm. F Schematic diagram of experimental design. G Top 10 Gene Ontology (GO) enrichment terms from H3K4me3 ChIP-seq in contralateral microglia one week after CMI induction. H Top 10 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment components from H3K4me3 ChIP-seq in contralateral microglia one week after CMI induction. Quantitative analysis of H3K4me3 ChIP-qPCR on TNF-α (I), IL-6 (J), IL-1β (K), and IL-10 (L) promoters in contralateral microglia after CMI induction, n = 12 mice per group. One-way ANOVA. F4, 55 = 7.884, p < 0.0001; Sham vs CMI1w: p = 0.0434; Sham vs. CMI 2w: p < 0.0001; Sham vs. CMI 3w: p = 0.0218; Sham vs. CMI 4w: p = 0.0256 (I); F4, 55 = 5.031, p = 0.0016; Sham vs. CMI1w: p = 0.0091; Sham vs. CMI 2w: p = 0.9744; Sham vs. CMI 3w: p = 0.9477; Sham vs. CMI 4w: p = 0.9488 (J); F4, 55 = 11.98, p < 0.0001; Sham vs. CMI1w: p < 0.0001; Sham vs. CMI 2w: p = 0.0037; Sham vs. CMI 3w: p = 0.7670; Sham vs. CMI 4w: p = 0.0183 (K); and F4, 55 = 1.38, p = 0.253; Sham vs CMI1w: p = 0.6866; Sham vs. CMI 2w: p = 0.5709; Sham vs CMI 3w: p = 0.2671; Sham vs. CMI 4w: p = 0.2671 (L) with Tukey’s correction. Data are presented as mean ± standard deviation (SD), n.s., non-significant, *p < 0.05, **p < 0.01, and ***p < 0.001.
