Fig. 4: Itch knockdown leads to an accumulation of mitochondrial cholesterol. | Cell Death & Disease

Fig. 4: Itch knockdown leads to an accumulation of mitochondrial cholesterol.

From: The E3 ubiquitin ligase Itch regulates death receptor and cholesterol trafficking to affect TRAIL-mediated apoptosis

Fig. 4

A Transmission electron micrographs illustrating the morphology of control and Itch KD cells. Scale bars, 5 µm. B Electron micrographs illustrating the morphology and size of mitochondria in control and Itch KD cells. Scale bar, 1 µm for all images. C Quantification of mitochondrial width from 25 cells. Mean ± SD. D Confocal images illustrating the accumulation of free cholesterol by Filipin-III staining together with outer mitochondrial membrane marker TOM20. Scale bar, 5 µm. E Tukey box plot displaying the Pearson colocalization co-efficient of TOM20 and Filipin staining to compare levels of mitochondrial cholesterol in control and Itch KD cell lines. N = 14 per group. F Western blot analysis of cell lysates showing the expression of nuclear SREBP2 (cleaved) protein in the Itch KD compared to control. Statistical significance was measured by Student’s t test. ***p < 0.001, ****p < 0.0001.

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