Fig. 4: PHLPP2 is regulated by IKKα and IKKβ in TRIM22-overexpressed cells.
From: TRIM22 induces cellular senescence by targeting PHLPP2 in hepatocellular carcinoma
A IP using anti-PHLPP2 in TRIM22-overexpressed HepG2 cells. Immunoprecipitates were analyzed by Western blotting using the indicated antibodies. B IP was performed using anti-PHLPP2 antibody in TRIM22-overexpressed HepG2 cells. After IP, lysates were treated with λ-PPase and analyzed by Western blotting using the indicated antibodies. C Workflow of strategy used to identify kinase candidates that interact with and phosphorylate PHLPP2. D STRING analysis of PHLPP2-interacting kinases. E Western blot analysis of kinases in TRIM22-overexpressed HepG2 cells. F HepG2 cells were transfected with siRNA targeting each indicated kinase. After 48 hrs, the cells were harvested and analyzed by Western blotting using the indicated antibodies. G IP using anti-IKKα (left) or anti-IKKβ (right) in TRIM22-overexpressed HepG2 cells. Immunoprecipitates were analyzed by Western blotting using the indicated antibodies.
