Fig. 6: TRIM22 and PHLPP2 levels are inversely correlated in HCC and paired normal patient tissues.
From: TRIM22 induces cellular senescence by targeting PHLPP2 in hepatocellular carcinoma
A, B Comparison of TRIM22 and PHLPP2 mRNA levels in HCC and paired normal tissue samples from ICGC-LIRI database [Normal (N), n = 177; Tumor (T), n = 212. Paired samples, n = 177 (A)] (TRIM22 mRNA: unpaired two-tailed t-test, ***P < 0.0001, t = 8.059; paired two-tailed t-test, ***P < 0.0001, t = 9.223; PHLPP2 mRNA: unpaired two-tailed t-test, ***P < 0.0001, t = 4.756; paired two-tailed t-test, ***P < 0.0001, t = 5.125) and TCGA-LIHC database [N, n = 50; T, n = 369. Paired samples, n = 50 (B)] (TRIM22 mRNA: unpaired two-tailed t-test, ***P < 0.0001, t = 5.485; paired two-tailed t-test, ***P < 0.0001, t = 5.693; PHLPP2 mRNA: unpaired two-tailed t-test, *P = 0.0105, t = 2.572; paired two-tailed t-test, ***P < 0.0001, t = 4.938). C Survival analysis between groups with different levels of TRIM22 and PHLPP2 in the TCGA-LIHC database. D Western blot analysis of TRIM22, P-IKKβ (Y188), and PHLPP2 in HCC and paired normal patient tissues. n = 30. Statistical analysis of the value intensity of TRIM22, PHLPP2, and P-IKKβ (Y188) normalized to those of Actin and Total IKKβ in patient tissues (TRIM22: paired two-tailed t-test, ***P = 0.0002, t = 4.206; P-IKKβ: paired two-tailed t-test, ***P = 0.0004, t = 4.003; PHLPP2: paired two-tailed t-test, ***P = 0.0002, t = 4.327). The protein levels were quantified by densitometry using the ImageJ software and normalized to the protein level of Actin or Total IKKβ. E IHC analysis of TRIM22 and PHLPP2 in HCC and paired patient tissues (left). n = 16. Scale bars, 50 μm. Correlation of TRIM22 and PHLPP2 IHC scores (right). r is the Spearman’s rank correlation coefficient.
