Fig. 1: The KRASG12C inhibitor ARS-1620 induces re-expression of KRAS and reactivation of ERK signal in lung cancer cells.
From: Hedgehog signalling is involved in acquired resistance to KRASG12C inhibitors in lung cancer cells
A, B and F H23 and C, D and G H358 lung cancer cells were treated with 10 μM ARS-1620 for the indicated times. Time-dependent changes in ERK phosphorylation and accumulation of KRAS-GTP in H23 (A, B) and H358 cells (C, D) were determined by western blot analysis. Time-dependent changes in KRAS mRNA levels in H23 (F) and H358 cells (G) were determined by qRT-PCR. Fold-change in expression level was calculated relative to the values at time 0 for each cell. The graphs are mean ± standard deviation (n = 3 independent experiments) (one-way ANOVA, **P < 0.01; ***P < 0.001). E Representative plots of flow cytometry using propidium iodide staining for cell cycle analysis of H358 cells. Graphs represent the mean ± standard deviation (n = 3 independent experiments).
