Fig. 6: Nuclear translocation of ATF3 in Smad4-overexpressing neuro-2a cells.
From: Transmembrane serine protease 6, a novel target for inhibition of neuronal tumor growth
Neuro-2a cells were transfected with pcDNA3.1 (Vector group) or pcDNA3.1-Smad4-3Flag (Smad4 group) plasmids. A Levels of total FLAG, Smad4, and ATF3, as assessed by western blot analysis (n = 4). B Quantification of total Smad4 and ATF3 expression from the experiment shown in A. C The levels of Smad4 and ATF3 in the cytoplasm and nucleus were detected by western blot analysis (n = 4). D, E Quantification of Smad4 and ATF3 levels in the cytoplasm and nucleus in the experiment shown in E. Each sample’s relative expression level was calculated as described in the legend of Fig. 1D. F The subcellular localization of ATF3 in neuro-2a cells was examined by immunofluorescence (Scale bar = 20 μm). G The interaction between FLAG (Smad4) and ATF3 was examined by immunoprecipitation. Data are expressed as the mean ± SD. ∗p < 0.05.∗∗p < 0.01.***p < 0.001.
