Fig. 2: 5-Aza elevates VDR expression by decreasing global DNA methylation in human PDAC cells. | Cell Death & Disease

Fig. 2: 5-Aza elevates VDR expression by decreasing global DNA methylation in human PDAC cells.

From: Epigenetic priming targets tumor heterogeneity to shift transcriptomic phenotype of pancreatic ductal adenocarcinoma towards a Vitamin D susceptible state

Fig. 2: 5-Aza elevates VDR expression by decreasing global DNA methylation in human PDAC cells.

A Dot blot analysis for spotting of total DNA stained with anti-5mC (Upper) and methylene blue (Bottom) showed that 5-Aza decreases global DNA methylation in MiaPaca2 and Panc1 cells. B Immunoblots for whole cell lysates showed that 5-Aza elevates VDR expression irrespective of H3K27ac levels in MiaPaca2 and Panc1 cells. C 72-hour proliferation assay of MiaPaca2 and Panc1 cells with indicated treatment. Data are plotted as Mean ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test. n.s. no significance or P > 0.05; *P < 0.05 and ****P < 0.001. D Immunoblots for whole cell lysates showed that 5-Aza elevates VDR expression in a dose-dependent manner in MiaPaca2 rather than Panc1 cells. ND not detected or not determined.

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