Fig. 7: Combined inhibition of multiple pathways is required for altering the magnitude of killing mediated by CAR T cells. | Cell Death & Disease

Fig. 7: Combined inhibition of multiple pathways is required for altering the magnitude of killing mediated by CAR T cells.

From: Decoding the mechanisms of chimeric antigen receptor (CAR) T cell-mediated killing of tumors: insights from granzyme and Fas inhibition

Fig. 7

A, C, E Time-dependent cytotoxicity plot of 19–41BB CAR T cells treated with cytotoxic inhibitors against tumor cell lines. Cytotoxicity percentage was corrected by subtracting the spontaneous target death percentage via control wells. The red data points represent T cells without treatment, the green data points represent T cells incubated with GZMB and FasL inhibitors, and the black data points represent T cells incubated with GZMA and GZMB inhibitors. Asterisks represent statistical testing between the bottom curve and the timepoints corresponding to the color of the asterisk. B, D, F End point microscopy images from each condition. Tumor cells are green, the death marker is red, and dead tumors appear as yellow. The scale bar represents 300 μm. * Cytotoxicity plots show the mean percentage of three replicate wells (N = 3) and the error bars show the SEM. Statistical testing for the cytotoxicity assay was performed at each time point using Tukey’s method for multiple comparisons.

Back to article page