Fig. 6: POLB is post-translationally regulated by CALR. | Cell Death & Disease

Fig. 6: POLB is post-translationally regulated by CALR.

From: DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1

Fig. 6

A Heatmap of genes derived from label-free proteomics analyses. B A schematic model of the bioinformatics screening for Calr. C Co-IP assays in the liver lysates from ZT1 and ZT13 mice. Immunoblots of precipitated proteins were performed by using indicated antibodies. n = 3. D Co-IP assays in mouse PHs. Immunoblots of precipitated proteins were performed by using indicated antibodies. n = 3. E Western blot analyses of POLB and CALR expression in mouse PHs transfected with indicated amounts of plasmids encoding mouse Calr. n = 3. F The CALR-induced degradation of POLB protein were assessed by CHX chase experiments. n = 3. G Ubiquitination of POLB protein were detected by Western blot. All values are presented as the mean ± SD.

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