Fig. 5: CYLD promoted ferroptosis in prostate cancer by activating ACSL4/TFRC.
From: CYLD regulates cell ferroptosis through Hippo/YAP signaling in prostate cancer progression
A Heatmap of ferroptosis-associated factors regulated by CYLD knockdown in DU145 sublines by RNA-seq. B, C Analysis of linear correlation between the expression level of CYLD mRNA and the expression level of ACSL4, TFRC mRNA from TCGA. D RT-PCR analysis of the expression of six ferroptosis-associated factors after knocking-down CYLD in DU145 cells. 18 S was applied as the endogenous control (N = 3). E Western blot analysis of ACSL4 and TFRC expression levels in DU145/shCon and DU145/shCYLD sublines. F RT-PCR analysis of the expression of six ferroptosis-associated factors in PC-3 sublines. G Western blot analysis of ACSL4 and TFRC expression levels in PC-3/NC and PC-3/CYLD sublines. H representative pictures and IHC analysis of the correlation between CYLD protein expression and ACSL4, TFRC protein expression in PCa specimens (n = 20). (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).
