Fig. 6: CYLD promoted ferroptosis in PCa cells by regulating Hippo/YAP pathway.
From: CYLD regulates cell ferroptosis through Hippo/YAP signaling in prostate cancer progression
A after overexpression of YAP in DU145/shCYLD sublines, the protein expression of ACSL4 and TFRC were examined by Western blot analysis. B Cell viability of DU145/shCYLD sublines after YAP overexpression and RSL3 treatment or ferrostatin-1 (Fer-1; 5 μM) treatment as indicated. C Lipid peroxidation measurement in DU145/shCYLD sublines after YAP overexpression and RSL3 treatment or ferrostatin-1 (Fer-1; 5 μM) for 6 h. D Results of PI staining of DU145/shCYLD sublines after YAP overexpression and RSL3 treatment or ferrostatin-1 (Fer-1; 5 μM) for 12 h. E after knocking-down YAP by siRNAs in PC-3/CYLD sublines, the protein expression of ACSL4 and TFRC were examined by Western blot analysis. F Cell viability of PC-3/CYLD sublines after siYAP transfection and RSL3 treatment or Ferrostatin-1 (Fer-1; 5 μM) treatment as indicated. G. Lipid peroxidation measurement in PC-3/CYLD sublines after siYAP transfection and RSL3 treatment for 12 h. H Results of PI staining of PC-3/CYLD sublines after siYAP transfection and RSL3 treatment for 20 h. (**p < 0.01; ***p < 0.001; ****p < 0.0001).
