Fig. 2: Peli1 deletion alters TAC-induced changes in fatty acid gene expression profiles.
A–G 8-week-old WT and Peli1KO mice were randomly subjected to TAC or sham surgery for 4 weeks. RNA-seq analysis was performed using mRNA extracted from left ventricular myocardium. A Venn diagram of differentially expressed genes that changed more than 2-fold in the WT TAC group compared to the WT sham group and KO TAC group compared to the WT TAC group (n = 3). B Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of these 656 genes. C Line graph of GO_ Biological Process (GO_P) analysis of genes under the category “lipid metabolic process” (red rectangle) in (B). D Heat map showing the variation of differentially expressed genes under the catabolic categories in (C). E, F Western blot analysis of protein levels of HNF4α, GAPDH as the loading control (n = 6). G qRT-PCR was performed to analyze the mRNA levels of CPT1b, CPT2, CD36, CrAT, ACAT1 and Acadm (n = 8 for WT sham group, n = 9 for WT TAC group, n = 6 for KO sham group and n = 10 for KO TAC group). H–J 8-week-old Peli1f/f and Peli1cKO mice were subjected to TAC or sham surgery for 4 weeks. H, I Western blot analysis of protein levels of HNF4α, GAPDH as the loading control (n = 3). J qRT-PCR was performed to analyze the mRNA levels of CPT1b, CPT2, CD36, CrAT, ACAT1 and Acadm (n = 6). Values are mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA followed by Tukey’s test).
