Fig. 2: IGF2BP3 impacts on cell viability and proliferation in glioma cells. | Cell Death & Disease

Fig. 2: IGF2BP3 impacts on cell viability and proliferation in glioma cells.

From: Depletion of the N6-Methyladenosine (m6A) reader protein IGF2BP3 induces ferroptosis in glioma by modulating the expression of GPX4

Fig. 2

A U87, U251, and HS683 cells were infected with shNC, shIGF2BP3-1, and shIGF2BP3-2 lentivirus. The IGF2BP3 knockdown efficiency was verified by RT-qPCR. Paired two-tailed t-test was used to compare the results (n = 3). B CCK-8 showing cell viability of U87, U251, and HS683 cells with IGF2BP3 knockdown compared to control cells. Paired two-tailed t-test was used to compare the results (n = 3). C Colony formation assay showing effects of IGF2BP3 knockdown on clonogenicity in U87, U251, and HS683 cells. Paired two-tailed t-test was used to compare the results (n = 3). D Cell cycle analysis showing IGF2BP3 knockdown led to S-phase arrest in U87 cells. Multiple t-test was used to compare the results (n = 4). E Cell apoptosis analysis demonstrating an increase in cell apoptosis and cell death upon IGF2BP3 knockdown in U87 cells. Adding of apoptosis inhibitor zVAD-fmk resulted in partial mitigation of the apoptosis triggered by IGF2BP3 knockdown (Multiple t-test, n = 3). Data are presented as the mean ± standard deviation (SD) from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05.

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