Fig. 6: GITRL suppresses the differentiation of liver progenitor cells into hepatocytes, and GITR further hinders this process by reducing the Wnt/β-catenin pathway. | Cell Death & Disease

Fig. 6: GITRL suppresses the differentiation of liver progenitor cells into hepatocytes, and GITR further hinders this process by reducing the Wnt/β-catenin pathway.

From: GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice

Fig. 6: GITRL suppresses the differentiation of liver progenitor cells into hepatocytes, and GITR further hinders this process by reducing the Wnt/β-catenin pathway.

A Representative bright-field microscopy images or EpCAM immunofluorescence images of the liver progenitor organoids from Sox9Cre-ERRosaTdTomatoTnfsf18+/+ mice or Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox mice. B The mRNA transcript levels of GITRL, EpCAM, PCNA, Cyclin D, CK19, AFP, and ALB in the Sox9Cre-ERRosaTdTomatoTnfsf18+/+ or Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox liver progenitor organoids. C The mRNA transcript levels of EpCAM and ALB in the GITR-Fc-exposed Sox9Cre-ERRosaTdTomatoTnfsf18+/+ or Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox liver progenitor organoids. D Principal component analysis (PCA) of GITR-Fc- or IgG-exposed Sox9Cre-ERRosaTdTomatoTnfsf18+/+ or Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox liver progenitor organoids. E GSEA of GITR-Fc-exposed Sox9Cre-ERRosaTdTomatoTnfsf18+/+ liver progenitor organoids v.s. their IgG-exposed organoids and GITR-Fc-exposed Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox liver progenitor organoids v.s. their IgG-exposed organoids. F GSEA revealed suppression of Wnt/β-catenin signalling in GITR-Fc-exposed Sox9Cre-ERRosaTdTomatoTnfsf18+/+ liver progenitor organoids v.s. their IgG-exposed organoids. G The mRNA transcript levels of Axin1, Fzd1, and Skp2 in the GITR-Fc-exposed Sox9Cre-ERRosaTdTomatoTnfsf18+/+ or Sox9Cre-ERRosaTdTomatoTnfsf18flox/flox liver progenitor organoids and their IgG-exposed control organoids.

Back to article page