Fig. 2: Fumarate induced MIR4435-2HG expression by H3K4me3 modification. | Cell Death & Disease

Fig. 2: Fumarate induced MIR4435-2HG expression by H3K4me3 modification.

From: Fumarate induces LncRNA-MIR4435-2HG to regulate glutamine metabolism remodeling and promote the development of FH-deficient renal cell carcinoma

Fig. 2

A The representative images of cellular immunofluorescence to detect the MIR4435-2HG localization in FH-deficient cancer cells. B The specific enrichment of H3K4 methylation and H3K27 acetylation peaks in the promoter region of MIR4435-2HG according to the UCSC Genome Bioinformatics Site (http://genome.ucsc.edu/). C The expression of common demethylases and deacetylase in HLRCC tissues. B The H3K4me1, H3K4me3, H3K27Ac enrichment in MIR4435-2HG. D The western-blot analysis was performed to determine the H3 methylation and acetylation level in cell lines. E H3K4me3 level was analyzed after MMF treatment. F qPCR was performed in HK2, pFH and UOK262 cells treated with MMF. G–I ChIP assay was performed to determine the H3K4me3 level in MIR4435-2HG promoter region. *p < 0.05, **p < 0.01, ***p < 0.001.

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