Fig. 2: Gasdermin E, but not GSDMD, was activated in mouse renal IRI. | Cell Death & Disease

Fig. 2: Gasdermin E, but not GSDMD, was activated in mouse renal IRI.

From: CHOP-mediated Gasdermin E expression promotes pyroptosis, inflammation, and mitochondrial damage in renal ischemia-reperfusion injury

Fig. 2

A Representative immunofluorescence image of GSDME and GSDMD in human renal cortex (Normal). Red fluorescence represents GSDME and GSDMD, and Green fluorescence represents Lotus Tetragonolobus Lectin (LTL), proximal tubular epithelial cell marker. n = 6/group Norma Scale bar: 100 μm; Scale bar: 20 μm(magnification). B Western blotting analysis of GSDME and GSDMD expression in human renal cortex (Normal). n = 14/group. C Quantitative polymerase chain reaction (qPCR) analysis of the gene expression relative to GSDMD as well as GSDME in human renal cortex (Normal). n = 14/group. D, E Representative immunofluorescence image of GSDME and cleaved-caspase3 were expressed in mice kidney. Red fluorescence represents GSDME and cleaved-caspase3. F Representative immunofluorescence image of GSDMD in mice kidney, Red fluorescence represents GSDMD. n = 6/group Scale bar: 100 μm. G Western blotting analysis of GSDME, GSDME-N, Caspase3, Cleaved-Caspase3, NLRP3, Caspase1, Cleaved-Caspase1, Caspase8, Cleaved-Caspase8, Caspase11, Cleaved-Caspase11, GSDMD, GSDMD-N expression in mice kidney. n = 6/group. For all panels, p value was determined by unpaired two-tailed Student’s t test or one-way ANOVA with Bonferroni post hoc test for multiple comparisons. Data are expressed as mean ± SEM. Quantification on the blots derive from samples of the same experiment and gels/blots were processed in parallel. ns not significant p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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