Fig. 6: Mysm1 knockdown promotes NSC proliferation in the brains of aged mice.

Immunofluorescence (left) for Nestin and Mysm1 in the SGZ (A) or SVZ (B) of 4-week-old, 10-week-old, and 15-month-old WT mice. The boxed areas are shown in detail at the bottom. Percentage (right) of Mysm1⁺ Nestin⁺ NSCs among total Nestin⁺ NSCs per SGZ (A) or SVZ (B) section of 4-week-old, 10-week-old, and 15-month-old WT mice (n = 5). Immunofluorescence (left) for Gfap and Mysm1 in the SGZ (C) or SVZ (D) of 4-week-old, 10-week-old, and 15-month-old WT mice. The boxed areas are shown in detail at the bottom. Percentage (right) of Mysm1⁺ rGfap⁺ NSCs among total rGfap⁺ NSCs per SGZ (C) or SVZ (D) section of 4-week-old, 10-week-old, and 15-month-old WT mice (n = 5). E Schematic of coronal and sagittal sections showing the location of AAV injection in the SVZ. Brains were analyzed 2 h after EdU injection. F Immunofluorescence (left) for Nestin and Ki67 in the SVZ of AAV-Con and AAV-ShMysm1 mice. The boxed areas are shown in detail as insets. Percentage (right) of Ki67⁺ Nestin⁺ NSCs among total Nestin⁺ NSCs per SVZ section of AAV-Con and AAV-ShMysm1 mice (n = 4). G Immunofluorescence (left) for Nestin and EdU in the SVZ of AAV-Con and AAV-ShMysm1 mice. Percentage (right) of EdU⁺ Nestin⁺ NSCs among total Nestin⁺ NSCs per SVZ section of AAV-Con and AAV-ShMysm1 mice (n = 4). H Immunofluorescence (left) for Gfap and Ki67 in the SVZ of AAV-Con and AAV-ShMysm1 mice. The boxed areas are shown in detail as insets. Percentage (right) of Ki67⁺ rGfap⁺ NSCs among total rGfap⁺ NSCs per SVZ section of AAV-Con and AAV-ShMysm1 mice (n = 4). I Immunofluorescence (left) for Gfap and EdU in the SVZ of AAV-Con and AAV-ShMysm1 mice. Percentage (right) of EdU⁺ rGfap⁺ NSCs among total rGfap⁺ NSCs per SVZ section of AAV-Con and AAV-ShMysm1 mice (n = 4). Data are presented as the mean value ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****P < 0.0001. Scale bar represents 50 μm (A, B, C, D, F, G, H, and I).