Fig. 2: DCK-inactivated PDAC cells have a higher oxidative phosphorylation. | Cell Death & Disease

Fig. 2: DCK-inactivated PDAC cells have a higher oxidative phosphorylation.

From: Deoxycytidine kinase inactivation enhances gemcitabine resistance and sensitizes mitochondrial metabolism interference in pancreatic cancer

Fig. 2

a Mito stress test profiles demonstrating an increase in oxygen consumption rate (OCR) in DCK#10 under basal condition, followed by the injection of oligomycin (1.5 μmol/L), FCCP (1 μmol/L), and rotenone + antimycin A (0.5 μmol/L). b Bar graphs revealing increased basal respiration, maximal respiration, and ATP production rate in DCK#10 cells in (a). c Glycolysis stress test profiles demonstrating a decrease in extracellular acidification rate (ECAR) in DCK#10 under basal condition, followed by the injection of glucose (10 mmol/L), oligomycin (1 μmol/L), and 2-deoxyglucose (50 mmol/L). d Bar graph demonstrating reduced glycolysis, glycolytic capacity, and glycolytic reserve in DCK#10 cells in (d). e Energy map revealing reduced DCK#10 cell energetic efficiency under both baseline and stressed conditions. f Reduced metabolic potential of DCK#10 cells compared to control NT1 cells under energetically stressed conditions. NT1, n = 10; DCK#10, n = 8 for (e) and (f). Data are expressed as mean ± standard deviation from three independent triplicate experiments. *P < 0.05; **P < 0.01; ***P < 0.001.

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