Fig. 6: MST1 overexpression ameliorates myeloid-specific TSC1 deficiency-mediated liver damage in I/R-induced liver injury. | Cell Death & Disease

Fig. 6: MST1 overexpression ameliorates myeloid-specific TSC1 deficiency-mediated liver damage in I/R-induced liver injury.

From: Loss of macrophage TSC1 exacerbates sterile inflammatory liver injury through inhibiting the AKT/MST1/NRF2 signaling pathway

Fig. 6

BMMs from TSC1M-KO mice were transfected with lentivirus expressing MST1 (Lv-MST1) or GFP control (Lv-GFP) and adoptively transferred into TSC1M-KO mice. A The serum ALT levels from the indicated groups (n = 4–6 mice/group). ***p < 0.001. B Representative H&E staining and Suzuki’s score of liver sections (n = 4–6 mice/group), Scale bars = 250 μm. ***p < 0.001. C Representative immunohistochemistry staining and quantification of Ly6G+ neutrophils in liver sections (n = 4–6 mice/group), Scale bars = 150 μm. **p < 0.01. D Western blot analysis of p-MST1, NRF2, and TLR4. β-actin served as an internal control. Data are representative of three experiments. E Quantitative RT-PCR-assisted detection of TNF-α, IL-1β, IL-6 and TGF-β expression. Mean ± SD (n = 4–6 samples/group). **p < 0.01.

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