Fig. 4: Inhibition of SIRT3 promoted RSL3-induced U251 and GBM4# cell ferroptosis in vitro.
From: Targeting SIRT3 sensitizes glioblastoma to ferroptosis by promoting mitophagy and inhibiting SLC7A11
A Inhibition of SIRT3 expression and activity in U251 cells. B Inhibition of SIRT3 expression and activity promoted RSL3-induced U251 cell death, which was partially rescued by DFO and totally rescued by NAC in comparison with control cells. C Inhibition of SIRT3 expression and activity promoted RSL3-induced U251 cell death, which was partially rescued by Fer-1. D Inhibition of SIRT3 expression and activity promoted LPO accumulation in U251 cells. E Inhibition of SIRT3 expression and activity promoted MDA accumulation in U251 cells. F Inhibition of SIRT3 expression and activity in GBM#4 cells. G Inhibition of SIRT3 expression and activity promoted RSL3-induced GBM#4 cell death. H Inhibition of SIRT3 expression and activity promoted LPO accumulation in U251 cells. I Inhibition of SIRT3 expression and activity promoted MDA accumulation in U251 cells. *p < 0.05, **p < 0.01, ****p < 0.0001.
