Fig. 3: Identification of SNHG26-associated proteins. | Cell Death & Disease

Fig. 3: Identification of SNHG26-associated proteins.

From: LncRNA SNHG26 promotes gastric cancer progression and metastasis by inducing c-Myc protein translation and an energy metabolism positive feedback loop

Fig. 3

A. RNA pull-down assay showing the proteins that bind with biotinylated SNHG26 in vitro by silver staining. B. Immunoblotting for specific associations of NCL with SNHG26 from RNA pull-down assays. C. RIP assays were performed using antibodies against NCL, and qPCR was used to detect SNHG26 enrichment. D. Immunoblotting for NCL in samples pulled down with full-length biotinylated-SNHG26 or truncated RNA motifs. E, F. Deletion mapping to identify the domains of NCL that bind to SNHG26. RIP analysis for SNHG26 enrichment in cells transiently transfected with full-length or truncated FLAG-tagged constructs.

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