Fig. 4: SNHG26 promotes protein synthesis in GC. | Cell Death & Disease

Fig. 4: SNHG26 promotes protein synthesis in GC.

From: LncRNA SNHG26 promotes gastric cancer progression and metastasis by inducing c-Myc protein translation and an energy metabolism positive feedback loop

Fig. 4

A–C Protein synthesis was measured in MKN-28 and HGC-27 GC cell lines with SNHG26 overexpressed or knocked down (OPP, green; DAPI, blue). Scale bars = 25 μM. D Quantification of Alexa 488 fluorescence intensity. E Western blotting was performed to assess protein synthesis in MKN-28 and HGC-27 cell lines overexpressing or knocking down SNHG26 treated with puromycin labeling, followed by detection with puromycin antibody. All data are from three independent experiments. The data are presented as the mean ± SD values (n = 3). *P <0.05; **P <0.01; ***P <0.001; ****P<0.0001.

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