Fig. 3: PTEN knockout induces hyperplasia of bronchial epithelial cells.

mRNA isolated from the lungs of EGFRL858R and EGFRL858R*PTEN-/- mice was used to measure the levels of cilia-related genes by qPCR, and then statistical analysis was performed by a t test; *p < 0.05, **p < 0.01, ***p < 0.001 A. The ciliated cells on the surface of bronchial cells were studied by Alcian blue staining B and IHC staining with anti-mucin antibodies C.