Fig. 4: PTEN decreases NR2F1 expression to inhibit DNAI2 expression in lung cancer. | Cell Death & Disease

Fig. 4: PTEN decreases NR2F1 expression to inhibit DNAI2 expression in lung cancer.

From: PTEN decreases NR2F1 expression to inhibit ciliogenesis during EGFRL858R-induced lung cancer progression

Fig. 4

mRNA levels of DNAI2, DNAI3 and MUC5AC in BEAS 2B cells with or without knockdown of PTEN and NR2F1 were measured by qPCR A. The levels of proteins B and mRNAs C in BEAS 2B cells with or without knockdown of PTEN and NR2F1 were measured by IB and qPCR. The mRNA levels of NR2F1 in the lungs of EGFRL858R and EGFRL858R*PTEN-/- mice and in PTEN knockdown BEAS 2B cells were measured by qPCR D, E. The p-AKT levels in the lungs of EGFRL858R and EGFRL858R*PTEN-/- mice were evaluated by IB F. The mRNA levels of DNAI2 G, a and DNAI3 G, b in BEAS 2B cells with or without PTEN knockdown and MK2206 treatment were measured by qPCR. The protein levels of NR2F1 G, c and p-AKT H in BEAS 2B cells with or without PTEN knockdown and MK2206 treatment were measured by IB. The recruitment of NR2F1 to the promoter of DNAI2 in BEAS 2B cells was evaluated by a chromatin immunoprecipitation (ChIP) assay H and (I, a, b). The transcriptional activity driven by the promoter of NR2F1 (2 kb) with or without PTEN knockdown was measured by luciferase activity assay I, c. The statistical analysis was performed by a t test; *p < 0.05, **p < 0.01, ***p < 0.001, ns: nonsignificant.

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