Fig. 1: IDH1 is a potential anti-HCC target for Scu.

A Chemical structure of Scu-B. B Schematic of the procedure for detecting binding events by HuProt^TM proteome microarray and IDH1 was identified as a direct cellular target of Scu. C IDH1 recombinant protein chip analysis identified IDH1 as a direct cellular target of Scu. D The expression profile of IDH1 in various tissues from the TCGA database. E Kaplan‒Meier plots of the disease-free survival (DFS) rates of groups with differential IDH1 expression in the liver cancer RNA-seq dataset. F IDH1 expression levels were decreased in tumor tissues (n = 64) compared with their matched adjacent nontumoral tissues (n = 32), as shown in representative immunohistochemical images and mean staining scores. G and H Growth curves of cells that were cultured in 24-well plates (1 × 10⁴ per well) for the indicated times under different oxygen concentrations. n = 3. I Colony formation assays of cells cultured in six-well plates (0.5 × 10⁴ per well) under different oxygen concentrations for two weeks. n = 3. J The growth of OE_IDH1 and OE_CON H22 cells in BALB/c mice was photographed 4 weeks after transplantation. n = 5. The dynamic change in K tumor volume, and the changes in L tumor weight in the subcutaneous model mice at four weeks after injection are shown. Scale bars, 200 μm. The data are mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001 compared to the control group.