Fig. 3: High expression of IDH1 increases the inhibitory effect of Scu on tumor cells under hypoxia.

A The 48-h proliferation curve of different tumor cell lines treated with Scu at different oxygen concentrations. n = 5. B Comparison of the 48-hour IC₅₀ values of Scu in different tumor cell lines treated with different oxygen concentrations. C Detection of the energy metabolism phenotype of tumor cells under different oxygen concentrations by a Seahorse energy metabolism instrument. n = 6. D Inhibitory effect of Scu on glycolysis in HepG2 cells subjected to hypoxia for 12 h. n = 6. E Cell viability assays of Sh_IDH1 and Sh_CON HepG2 cells treated with Scu for 48 h under hypoxia. n = 3. F Growth curves of Sh_IDH1 and Sh_CON HepG2 cells treated with Scu for 6 days under hypoxia. n = 3. G The growth of Sh_IDH1 and Sh_CON H22 cells in BALB/c mice was photographed after four weeks of 40 mg/kg/day Scu administration. n = 5. H Changes in tumor weight in H22 xenograft tumor models after four weeks of Scu administration are shown. I Relative expression of IDH1 and HIF1a proteins in different cells under hypoxia. n = 3. J Correlation analysis of IDH1 and HIF1a protein expression in different cells under hypoxia. n = 3. K Correlation analysis between IDH1 protein expression and sensitivity to Scu in different cells under hypoxia. n = 3. The data are mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001 compared to the control group.