Fig. 5: FBXO32 recognizes and interacts with PTEN through its C-terminus. | Cell Death & Disease

Fig. 5: FBXO32 recognizes and interacts with PTEN through its C-terminus.

From: FBXO32-mediated degradation of PTEN promotes lung adenocarcinoma progression

Fig. 5: FBXO32 recognizes and interacts with PTEN through its C-terminus.

A Wild-type FBXO32 and FBXO32 deletion mutants schematics. B Effect of FBXO32 deletion mutants on PTEN degradation in 293T cells as determined by western blotting. C 293T cells transfected with Myc-tagged PTEN and Flag-tagged wild-type FBXO32 or FBXO32 deletion mutants (FBXO32 ΔC, FBXO32 ΔF or FBXO32 ΔN) were treated with MG132 (10 μM) for 6 h and then harvested for immunoprecipitation and immunoblotting. D HEK293T cells were transfected with Myc-PTEN, HA-Ub and FLAG-FBXO32 WT or FBXO32 deletion mutants and incubated with MG132 after 24 h. The ubiquitination of PTEN was analysed.

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