Fig. 2: RUVBL1 regulates radiation resistance of breast cancer cells.

A Western blot for RUVBL1 and β-actin in MDA-MB-231 RUVBL1 cell line. B Western blot for RUVBL1 and β-actin in MDA-MB-231 shRUVBL1 cell line. C, D The stable cell lines underwent a series of radiation treatments at 0, 1, 2, 4, 6 and 8 Gy, followed by an MTT test (n = 3). E–H The same cell lines were subjected to radiation at 0, 1, 2, 4, and 6 Gy, prior to being cloned and evaluated for survival curve (n = 3). I Western blot for γ-H2AX and β-actin in MDA-MB-231 RUVBL1 cells with radiation (5 Gy). J Immunofluorescence for γ-H2AX in MDA-MB-231 RUVBL1 cells with radiation (5 Gy) (×10). Scale bars: 100 μm. K 2 × 10⁵ control or RUVBL1 MDA-MB-231 cells were subcutaneously injected into nude mice (n = 5). Since the 12th day, each group of nude mice were treated with radiation (Every 3 days, 3 Gy each time). Tumor growth curves were shown. Tumors (L) and tumor size (M) of mice were shown. Data presented as mean ± SD, *p < 0.05, statistical differences were assessed using two-tailed unpaired Student’s t-test (C, E, F, H, K, M). (*p < 0.05, **p < 0.01, ***p < 0.001).