Fig. 5: RUVBL1 regulates radiation resistance of breast cancer cells by DTL.

A Western blot for RUVBL1, DTL and β-actin in MDA-MB-231 control, RUVBL1, RUVBL1-shDTL#1 and RUVBL1-shDTL#2 stably expressed cells. B–D MDA-MB-231 control, RUVBL1, RUVBL1-shDTL#1 and RUVBL1-shDTL #2 cells were treated with radiation (0, 1, 2, 4, 6, 8 Gy), followed by MTT assay and clonogenic survival assay (n = 3). E 2 ×10⁵ MDA-MB-231 control, RUVBL1, RUVBL1-shDTL #1 and RUVBL1-shDTL #2 cells were subcutaneously injected into nude mice (n = 5). Since the twelfth day, each group of nude mice were treated with radiation (Every 3 days, 3 Gy each time). Tumor growth curves were shown. Tumors (F) and tumor size (G) of mice were shown. H Western blot assay was used to detect the expression of DTL in MDA-MB-231-RR. I The stable cell lines underwent a series of radiation treatments at 0, 4, and 6 Gy, followed by an MTT test (n = 3). J The MDA-MB-231-RR Control and shDTL cells were subjected to radiation at 0, 4, and 6 Gy, prior to being cloned and evaluated for survival curve (n = 3). K MDA-MB-231-RR cells transfected with shDTL were immunoprecipitated with RUVBL1 antibody and western blot analysis. Data presented as mean ± SD, *p < 0.05, statistical differences were assessed using two-tailed unpaired Student’s t-test (B, D, E, G, I, J). (*p < 0.05, **p < 0.01, ***p < 0.001).