Fig. 5: The interaction between p65 and GATA4.

A The luciferase reporter assay showed the effect of p50/p65 in HEK293T cells on MMP9 promoter with or without GATA4. Data are the means ± SDs from three determinations. **p < 0.01. B The luciferase reporter assay showed the effect of p65 in HEK293T cells on NF-κB response element of MMP9 promoter with or without GATA4. Data are the means ± SDs from three determinations. *p < 0.05; **p < 0.01. C Lysates in T47D cells were immunoprecitated using anti-p65 Ab. Purified cell extracts were tested by western blot with anti-GATA4 Ab. D HEK293T cells were co-transfected with GFP-p65 and Flag-GATA4, and lysates were immunoprecitated using anti-GFP Ab. Purified cell extracts were tested by western blot with anti-Flag Ab. E GST-pulldown assay showed the purified GST-p65 in E. coli BL21 had physical interaction with Flag-GATA4 in HEK293T cells. F T47D cells were stained with an anti-GATA4 (green) and an anti-p65 (red). Nuclei were stained with DAPI (blue), followed by visualization with confocal microscopy. G HEK293T cells were co-transfected with Myc-GATA4 or GATA4 truncated constitutions with GFP-p65, and lysates were immunoprecitated using anti-Myc Ab. Purified cell extracts were tested by western blot with anti-GFP Ab.