Fig. 4: Akt/mTOR pathway was inactivated in Nectin-4-MMAE-treated nectin-4 positive bladder cancer cells.

A, C Western blot analysis for the AKT/mTOR pathway showed the total protein levels and phosphorylation levels of Akt, mTOR, P70S6K, 4EBP1 in HT1376 and SW780 treated with Nectin-4-MMAE for the indicated time. B, D The quantification of p-Akt, p-mTOR, p-P70S6K, p-4EBP1 normalized by total Akt, mTOR, P70S6, and 4EBP1 in A, C.