Fig. 3: CAFs inhibit DDP-induced ferroptosis via secreting FGF5. | Cell Death & Disease

Fig. 3: CAFs inhibit DDP-induced ferroptosis via secreting FGF5.

From: Cancer-associated fibroblasts secrete FGF5 to inhibit ferroptosis to decrease cisplatin sensitivity in nasopharyngeal carcinoma through binding to FGFR2

Fig. 3

Human CAFs and NFs were isolated from NPC tissues and the adjacent normal tissues, respectively. A The expression levels of α-SMA and FAP in CAFs were detected by immunofluorescence. Green, α-SMA. Red, FAP. Blue, DAPI. Scale bar: 100 μm. B The protein levels of α-SMA, vimentin and FAP in NFs and CAFs were detected by western blot. C The protein level of FGF5 in fibroblasts was detected by western blot. D The secretion of FGF5 in fibroblasts was assessed by ELISA assay. NPC/HK1 and C666-1 cells were divided into following groups: control, DDP (5 μg/mL), DDP (5 μg/mL)+CM and DDP (5 μg/mL)+anti-FGF5 antibody. E Cell viability was monitored by CCK-8 assay. F The protein levels of GPX4 and SLC7A11 were detected by western blot. G–I The levels of MDA (G), Fe2+ (H) and GSH (I) in NPC cells were measured using commercial kits. J NPC cells were stained with BODIPY C11 and lipid peroxidation was assessed by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

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