Fig. 1: Identification of ferroptosis-specific surface proteins. | Cell Death & Disease

Fig. 1: Identification of ferroptosis-specific surface proteins.

From: Fatty acid-binding protein 5 is a functional biomarker and indicator of ferroptosis in cerebral hypoxia

Fig. 1: Identification of ferroptosis-specific surface proteins.

A Experimental strategy for identifying candidate biomarkers in ferroptosis-sensitive HT-1080 cells by surface biotinylation and protein mass spectrometry. B Lipid peroxidation induced by RSL3 (300 nM) treatment for 3 h in HT-1080 cells. Treated and untreated cells measured by BODIPY 581/591 C11 stain (BODIPY-C11). A typical flow histogram of three independent experiments is depicted. C Flow cytometry of untreated cells compared to short term (3 h) RSL3 (250 nM) treated HT-1080 cells. Selected populations designated by indicated gates and ratios in Table 1 (U1, untreated; ES, end-stage ferroptosis; P1, unshifted RSL3-treated; P2, FSC-shifted RSL3-treated). D Volcano plot of biotinylated candidate proteins (P2) identified by mass spectrometry with ≥2 peptides in relation to control population (U1). Selected significant proteins (Students t-test, above dotted line, p < 0.05) were investigated further.

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