Fig. 2: FABP5 is concentrated at cell surface during ferroptosis. | Cell Death & Disease

Fig. 2: FABP5 is concentrated at cell surface during ferroptosis.

From: Fatty acid-binding protein 5 is a functional biomarker and indicator of ferroptosis in cerebral hypoxia

Fig. 2: FABP5 is concentrated at cell surface during ferroptosis.

A Immunohistochemistry staining in normal human cerebral cortex sections showing endogenous expression using antibodies against CALML5, FABP5, CTSV, LGALS7, S100A14 as well as TfR1 identified previously in [28]). The data shown here are derived from the Human Protein Atlas [68]. B Normalized fluorescence intensity heat map of candidate biomarker proteins following treatment with equivalent death-inducing concentrations of different pharmacological agents in HT-1080 cells. C Validation of FABP5 antibody specificity. FABP5 overexpressing HT-1080 cells (FABP5 OE) with C-terminal FLAG tag compared to control (Ctrl) by fluorescence staining and Western blot. Results observed with two antibodies (see Supplemental Reagents Table) were indistinguishable. Right panel, FABP5 expression level was detected by whole membrane Western blot in given populations. D Timecourse of lipid peroxidation in HT-1080 as indicated by flow cytometry analysis of Bodipy C-11 fluorescence and FABP5 cell surface staining in unpermeabilized cells and confocal images of the same cells.

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