Fig. 3: Human JK-GFP cells phagocytosis by zebrafish embryonic macrophages.
From: Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo
A Live imaging of JK-GFP cells injected in a zebrafish embryo at 30 h.p.f. (arrows indicate two cells). Starting from 33 min post transplantation, some green cells appear fragmented (C, D arrows). D Inset shows a high magnification of fragmented cells at 61 min (arrows). Each frame is a maximum projection of 3 planes apart 1 μm. See also Video 4. E–J Macrophages recruitment in a 4-d.p.f. Tg (mpeg1:mCherry) zebrafish embryo after injection with PBS (E–G) or with JK-GFP cells (H–J) (arrow). Merged picture (J) shows the accumulation of macrophages around JK-GFP cells. K Live imaging of mCherry-macrophages in a 4-d.p.f. embryo, 2 days after transplantation of JK-GFP cells. High magnification reveals the phagocytosis of green human cells engulfed by red host macrophages. Each frame is a maximum projection of 5 planes apart 0.8 μm.See also Video 5. Scale bars: (A–D): 25 µm, (E–J): 250 µm, (K): 20 µm. Time code in minutes.
