Fig. 4: CD34-GFP cells phagocytosis by zebrafish embryonic macrophages.
From: Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo
A, B Z-stack confocal imaging of mCherry-expressing macrophage behavior in a zebrafish embryo 2 h post transplantation (h.p.T.) of CD34-GFP, reveals the accumulation of macrophages around green cells and their phagocytosis (inset C, D and E). A Bright field imaging of caudal hematopoietic tissue in the tail region at 30 h.p.f., merged with confocal fluorescent imaging of CD34-GFP cells and mCherry-expressing macrophages (B). C–E” 3 successive z-stacks confocal planes apart 0.8 μm of phagocytosis of CD34-GFP cells by mCherry-expressing macrophage (arrows). See also Video 6. A aorta, CV caudal vein, NC notochord. Scale bars: A, B: 25 µm; C–E”:10 µm.
