Fig. 1: PTENα/β-NTE binds to WDR5.
From: The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif
A Domain structure of human PTENα/β-NTE and WDR5. PTENα/β-NTE consists of a WDR5 binding motif, a nuclear localization signal (NLS). WDR5 consists of seven WD40 repeats. The WDR5 binding sequence, namely SSSRRSS, under the PTENα/β-NTE was identified by this study. B ITC binding curves for the titration of PTENα-NTE116–148 or PTENα-NTE1–173 to WDR522–334 by iTC-200 microcalorimeter (MicroCal, Inc.). Kd: dissociation constants (μM); ITC data shown are representative of two independent experiments and all Kd values were calculated from a single measurement and errors were estimated by fitting curve.
