Fig. 6: Seipin deficiency compromised LDs dynamics & sphingolipid metabolism in OPC differentiation. | Cell Death & Disease

Fig. 6: Seipin deficiency compromised LDs dynamics & sphingolipid metabolism in OPC differentiation.

From: Seipin deficiency-induced lipid dysregulation leads to hypomyelination-associated cognitive deficits via compromising oligodendrocyte precursor cell differentiation

Fig. 6

A Representative confocal images of NC and sh-Seipin OLN cells before and after differentiation. B Violin plots illustrate area, diameter, and number of LDs per cell in NC and sh-Seipin OLN cells before and after differentiation. Black lines, mean values of LDs area, diameter, or number. C Normalized mRNA levels of sphingolipid metabolism-related genes in NC and sh-Seipin OLN cells before and after differentiation. D–F MALDI-TOF MSI shows distribution of identified lipids (D), heatmap analysis of significantly changed lipids (E), and ion images of representative lipids (F) in the CC of adult Seipin-/- mice. undiff.: undifferentiated. diff.: differentiated for 24 h. Graph data were presented as mean ± s.e.m. with n = 3 mice/group (D–F). *P < 0.05, **P < 0.01, ***P < 0.001 (B, C Two-way ANOVA followed by Turkey-Kramer post hoc tests).

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