Fig. 7: RG treatment promoted OPC differentiation by restoring LDs dynamics and sphingolipid metabolism in OLN cells. | Cell Death & Disease

Fig. 7: RG treatment promoted OPC differentiation by restoring LDs dynamics and sphingolipid metabolism in OLN cells.

From: Seipin deficiency-induced lipid dysregulation leads to hypomyelination-associated cognitive deficits via compromising oligodendrocyte precursor cell differentiation

Fig. 7

A Representative images of differentiated NC, sh-Seipin (sh), and sh+RG cells. B Violin plots illustrate the area and number of LDs in differentiated NC, sh, and sh+RG groups. Black lines, mean values of LDs area and number. C Quantification of ORO staining. D Distribution of different-sized LDs in differentiated NC, sh, and sh+RG cells. E Normalized mRNA levels of sphingolipid metabolism-related genes in differentiated sh and sh+RG cells. F Percentage histogram of histological categories of differentiated NC, sh, and sh+RG cells. G Normalized Mag mRNA levels in differentiated NC, sh, and sh+RG cells. diff.: differentiated for 24 h. NC: OLN cells transfected with NC-shRNA. sh: OLN cells transfected with Seipin-shRNA. sh+RG: sh-Seipin cells treated with RG. Graph data were presented as mean ± s.e.m. n > 150 cells (B, D, and F). *P < 0.05, **P < 0.01, ***P < 0.001 (B, C, and G One-way ANOVA followed by Bonferroni’s post hoc test. E unpaired t-test).

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