Fig. 2: Reg4 deficiency aggravates caerulein-induced pancreatic injuries.
From: Reg4 deficiency aggravates pancreatitis by increasing mitochondrial cell death and fibrosis
A Mice models of acute pancreatitis (AP) and chronic pancreatitis (CP). AP was induced in mice with 8 hourly injections of caerulein in Reg4 knockout (Reg4−/−) mice and wild-type (Wt) mice. The animals were sacrificed for analysis 6, 24, or 30 h after the initial caerulein injection. CP was then established by repeated episodes of acute caerulein-induced pancreatitis over 4 weeks. Mice were killed 2, 4, 6 or 8 weeks after the first injection. B, C Immunofluorescence (IF) staining of pancreatic sections for Amylase (red) and glucagon (green) + insulin (red) during pancreatitis (for each group, n ≥ 4; t = 6 h, 24 h, 2 weeks, 4 weeks, or 6 weeks). D Serum activities of amylase in Reg4−/− and Wt mice injected with caerulein were determined at the indicated time points (for each group, n ≥ 4; unpaired, two-tailed Student’s t-test with or without Welch’s correction analysis for (D); ns not significant, *P < 0.05, **P < 0.01).
