Fig. 3: Reg4 loss reduces regeneration of the ductal and acinar cells during pancreatitis.

A IF staining of pancreatic sections for amylase (green) and CK19 (red) during pancreatitis (for each group, n = 4; t = 24 h, 2 weeks, 4 weeks, or 6 weeks). B Quantification of the acinar cell area and ductal cell number per field in (A). C IF staining of the pancreatic sections for Ki67 (green) and CK19 (red) during pancreatitis (for each group, n = 4; t = 24 h, 2 weeks, 4 weeks, or 6 weeks). D Quantification of the number of Ki67⁺-CK19⁺ cells per field in (C). E IF staining of the pancreatic sections for amylase (green) and Ki67 (red) during pancreatitis (n = 4 for each group; t = 24 h, 2 weeks, 4 weeks, or 6 weeks). F Quantification of the Ki67⁺-amylase⁺ cells per field in (E); unpaired, two-tailed Student’s t-test with or without Welch’s correction analysis for (B, D, and F) (ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001).