Fig. 5: rReg4 treatment increases cell regeneration and autophagy during pancreatitis.
From: Reg4 deficiency aggravates pancreatitis by increasing mitochondrial cell death and fibrosis
A Western-blot analysis for amylase, CK19, SOX9, and PCNA in the pancreas of Wt, Reg4−/− mice, and Reg4−/− mice with rReg4 treatment (n = 4 for each group, t = 24 h). B Quantification of (A). GAPDH was used as an internal reference. C Western-blot analysis for p-mTOR, mTOR, p-AMPK, AMPK, P62, and LC3 in the pancreas of Wt, Reg4−/− mice, and Reg4−/− mice with rReg4 treatment (n = 4 for each group, t = 24 h). D Quantification of the panel C. E Representative images of Immunofluorescence analysis for LC3 in the sections of the pancreas of Wt, Reg4−/− and Reg4−/− mice with rReg4 treatment mice. F Quantification of LC3 intensity in (E). Randomized one-way ANOVA for (B, D, and F) (ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001).
