Fig. 6: Ablation of Reg4 leads to an increase in caerulein-induced fibrosis. | Cell Death & Disease

Fig. 6: Ablation of Reg4 leads to an increase in caerulein-induced fibrosis.

From: Reg4 deficiency aggravates pancreatitis by increasing mitochondrial cell death and fibrosis

Fig. 6

A, B Representative images of Masson’s trichrome staining and immunofluorescence (IF) staining of α-SMA in the pancreas of Wt and Reg4−/− mice (n = 4 for each group; t = 2, 4, or 6 weeks). C Quantification of the fibrotic area using Masson’s trichrome staining of images in (A). D Quantification of collagen content in the hydroxyproline assay in the pancreases of Wt and Reg4−/− mice (n ≥ 4 for each group; t = 2, 4, or 6 weeks). E Western-blot analysis for CXCL12 and CXCR4 in the pancreases of Wt and Reg4−/− mice after administration of caerulein for 1 week. F Quantification of panel E; GAPDH was used as the internal reference (n = 4); unpaired, two-tailed Student’s t-test with or without Welch’s correction analysis for C, D, and F (ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001).

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