Fig. 4: MEF PSDKO shows altered expression levels of mitochondrial transport proteins.

A SDS-PAGE of PS1, PS2, APP-CTFS, SNPH, Miro1, TRAK1 and TRAK2, Kif5 (A, B, C), IC 1, 2 in MEFs PSWT and PSDKO. Representative SDS PAGE of β-actin is shown as loading controls. MEF WT and MEF PSDKO samples were loaded in the same gels. The dashed line indicates that unrelated samples loaded between MEF WT and MEF PSDKO samples are not shown. Full-length western blots are provided in supplementary data. Quantitative graphs of SNPH (B), Miro1, TRAK1, TRAK2 (C), and Kif5 (A, B, C), Kif5 (low), and IC1, 2 (D) protein levels expressed as means ± SEM of MEF WT (set at 100%). Data were obtained from 4–5 independent experiments. *P < 0.05, **P < 0.01, and ns non-significant versus MEF PSWT using Mann–Whitney’s test.