Fig. 5: TREM2 regulates macrophage survival through the Akt/mTOR pathway.

a Volcano plot for WT, Trem2^−/− BMDM difference comparison. The horizontal coordinates indicate the logarithmic value of the difference in ploidy between the two subgroups, and the vertical coordinates indicate the negative Log10 value of the FDR of the difference between the two subgroups. Red (Trem2^−/− upregulated relative to WT expression) and blue (expression downregulated) points indicate a significant difference in gene expression (judged by FDR < 0.05, and more than two-fold difference), and black points indicate a lack of difference. b WT, Trem2^−/− KEGG enrichment bar graph of differential genes in BMDM. The vertical coordinate represents each signaling pathway, the horizontal coordinate represents the number of that signaling pathway as a percentage of the number of all differential genes, the darker the color the smaller the Q value, and the value on the bar represents the number of that signaling pathway and the Q value. c Heatmap of WT, Trem2^−/− BMDM for differential comparative clustering analysis. Each column in the graph represents a sample, each row represents a gene, and the expression of genes in different samples is indicated by different colors, with red colors indicating higher expression and blue colors indicating lower expression. d The differences in expression levels of mTOR, p-mTOR (ser2448), Akt, p-Akt (ser473), and p70S6K, key proteins of the mTOR signaling pathway, were detected by western blotting, and the bands analyzed in grayscale and statistically by using ImageJ software, n = 7–8. e Comparison of the CCK-8 results of WT and Trem2^−/− BMDM in vitro cultures after the addition of cyclocreatine days 3–5, n = 8. **P < 0.01, ****P < 0.0001.