Fig. 5: Kat2a promoted Tfrc and Hmox1 expression. | Cell Death & Disease

Fig. 5: Kat2a promoted Tfrc and Hmox1 expression.

From: Histone acetyltransferase Kat2a regulates ferroptosis via enhancing Tfrc and Hmox1 expression in diabetic cardiomyopathy

Fig. 5

A, B Tfrc and Hmox1 mRNA expression in heart tissues of control and DCM mice (N = 7 mice/group). C Tfrc and Hmox1 mRNA expression in NMVCs with or without HG treatment (N = 3 independent experiments). D The enrichment of H3K27ac and H3K9ac signals in the promoter region of Trfc and Hmox1 were visualized by ENCODE. E, H The enrichment of H3K27ac and H3K9ac signals in the promoter region of Trfc and Hmox1 were investigated by ChIP assay (N = 3 independent experiments). I The binding between Kat2a and Hmox1 promoter was verified by ChIP assay (N = 3 independent experiments). J The binding between Kat2a and Trfc promoter was verified by ChIP assay (N = 3 independent experiments). K The mRNA expression of Kat2a, Trfc, and Hmox1 in Kat2a overexpressed or depleted NMVCs (N = 3 independent experiments). L ChIP assay was used to assess the enrichment of H3K27ac, H3K9ac, and Kat2a in Trfc promoter in NMVCs (N = 3 independent experiments). M ChIP assay was used to assess the enrichment of H3K27ac, H3K9ac, and Kat2a in the Hmox1 promoter in NMVCs (N = 3 independent experiments). N Dual luciferase activity assays to analyze the fluorescence intensity of Kat2a-overexpressing and Kat2a-depleted NMVCs with the Trfc or Hmox1 promoter region (N = 3 independent experiments). Significance tested using: Student’s t-test (A, B) and One-way ANOVA (C, E–N). Statistical significance is shown as *p < 0.05, **p < 0.01, ***p < 0.001.

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