Fig. 2: Gsdmd deletion attenuates pyroptosis in asthmatic mice. | Cell Death & Disease

Fig. 2: Gsdmd deletion attenuates pyroptosis in asthmatic mice.

From: Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model

Fig. 2: Gsdmd deletion attenuates pyroptosis in asthmatic mice.

A Ultrathin lung sections were scanned using TEM to assess the pyroptosis of airway epithelium cells. The red arrow indicates the pyroptosis-related vacuolization structures, the yellow arrow indicates the mitochondria, the green arrow indicates the nuclear membrane. Scale bars: 2μm and 1μm as indicated. B The expression of Gsdmd detected by immunofluorescence staining in the lung tissue. Scale bars: 20 μm. C The secretion of IL-1β and IL-18 in the BALF of mice from the indicated group; n = 10 per group. D Representative images of lung sections from WT and Gsdmd−/− mice immunostained with anti-IL-18 and anti-IL-1β antibodies. Scale bars: 50 µm; n = 5 per group. E The protein level and quantification of F-Gsdmd, N-Gsdmd, IL-18, IL-1β, Caspase-1, and Caspase-11 were examined in the lungs of WT and Gsdmd−/− mice. β-actin is the internal control. n = 5 per group. No significance is indicated as NS. Results are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.

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